Laser Capture Microdissection

Palm MicroBeam

The PALM MicroBeam is the state-of-the-art device for modern microdissection and subsequent laser pressure catapulting. It is intended for the collection of precisely defined cells (living or fixed) or subcellular particles from all kind of samples as they are used in pathology, oncology, neurology or are needed for proteomics and cytogenetics as well as genetic engineering or other fields of biological, medical or pharmaceutical research.

This non-contact laser based micromanipulation system is designed to enable researchers to cut specific sections from sample slides and detach them from the surrounding material and then to catapult them into a collection cap. A pulsed ultraviolet laser is interfaced into a microscope and focused through objectives to a minimum spot size. The laser energy is concentrated only in this focal spot enabling a fine precise dissection. These sections are captured without the risk of contamination by the use of the Laser Pressure Catapulting process. Only the force of laser light is used to lift the samples against gravity into the collection cap.

Recharge Rates:

Two hour minimum	LA Biomed users:	Non LA Biomed users:
Assisted	$40.00/hour	$80.00/hour
Unassisted	$20.00/hour	$40.00/hour

· Equipment: PALM Microbeam pulsed UVa laser – more info

· Axiovert 200

· Filter Wheel – DAPI, FITC, Rhodamine

· Objective Lens: 10X and 40X

· Software: PALM RoboSoftware

Please contact Peng Xia pxia@labiomed.org to schedule an appointment.

Helpful Information:

· LCM principles.mht

· PALM_Supplies.mht

· High Quality RNA.pdf

· Protocols.pdf

Information provided by Carl Zeiss MicroImaging, Inc.