Confocal
Microscope
vi
Images of Candida from Dr. Filler’s Lab.
Double Click Image
. The Leica confocal SP2
microscope has many useful features. The microscope allows researchers
to focus on specific components inside a cell. Thin,
2-Dimensional, sequential, sections of a cell component may be scanned and
stacked together allowing the user to build 3-Dimensional images or
movies of interesting cellular structures.
Additionally, the confocal microscope allows researchers to show
co-localization of signals from different fluorochromes. Specimens can be
double- or triple-labeled for different cellular molecules or
structures; nucleic acids are stained red while proteins are labeled
green. These signals then can be
captured in different channels allowing individual or collective views. This
information can also be used to build 3-Dimensional images or movies.
Confocal Images
|
|
|
Images of
Candida from Dr. Filler
|
|
|
Image of a
Retina from Dr. Lue
Recharges:
|
Two Hour Minimum |
LA BioMed Users |
Non-LA BioMed Users |
|
Assisted Microscopy |
$40.00/hour |
$80.00/hour |
|
Unassisted Microscopy |
$20.00/hour |
$40.00/hour |
- Equipment: Leica SP2 Confocal
Leica DMXRE –
Upright
Leica DMIRE2 – Inverted with Environmental
Controls
- Lasers: Ar laser, excitation 488nm
Kr laser, excitation 568nm
HeNe laser
excitation 633nm
- Objective Lens: 10X, 40X oil, 63X water, and 100X oil
immersion
- Software: Leica Confocal Software version 2.00 and
Leica Lite
Please
contact jdedes@labiomed.org
to schedule an appointment.